Wednesday 19 December 2012

DRAQ7: a unique real-time cell health monitor


DRAQ7™ enables real-time toxicity and viability assays

  • DRAQ7™ is non-toxic to cells over several days exposure
  • DRAQ7™ does not alter dose-response profiles for anti-cancer agents
  • DRAQ7™ gives a sensitive real-time readout for cell death induced by hypoxia, starvation, drug treatment .. 
  • DRAQ7™ has full spectral compatibility with Hoechst 33342, FITC/GFP and the orange/red MMP probes

REFERENCE:
Akagi, J. et al. Real-time cell viability assays using a new anthracycline derivative DRAQ7.
Cytomery Part A. Early View - published online November 16, 2012 DOI: 10.1002/cyto.a.22228

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DRAQ7 Improves SP Analysis


DRAQ7™ permits a new kinetic understanding of SP analysis.  
  • Membrane-impermeant DRAQ7™ allows removal of interfering Hoechst signals coming from dead and damaged cells. 
  • DRAQ7™ is non-toxic, does not passively enter intact cells during the extended exposure times required and also allows sorting of the viable SP sub-populations. 
  • DRAQ7™ has no UV-excitation, avoiding PI's red emission overlap. 
  • Far-red DRAQ7™ permits multi-colour phenotypic SP analysis.
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REFERENCE:

Smith, P.J. et al. Kinetic analysis of intracellular Hoechst 33342-DNA interactions by flow cytometry: Misinterpretation of side population status?
Cytometry Part A. Early View - published online November 7, 2012 DOI: 10.1002/cyto.a.22224
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